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. 2019 Jun 20;47(14):7532–7547. doi: 10.1093/nar/gkz514

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Combined inactivation of both FANCI and FANCD2 is epistatic for bone marrow hematopoetic phenotypes. (A) Western blot analysis of FANCD2 and FANCI proteins in Fanci^−/−Fancd2^−/− knockout mice. (B) Fanci^−/−Fancd2^−/− mice are viable. The mice shown are 4 to 5 months old females. (C) Bone marrow progenitors from single KO mice or double KO mice are hypersensitive to Mitomycin C. Bone marrow cells were cultured in triplicates in methylcellulose medium containing Mitomycin C for 10 days in CFU-C assay and the number of hematopoietic colonies were counted. (D-I) Wild-type, Fancd2^−/−, Fanci^−/− or the double knockout Fancd2^−/−Fanci^−/−mice were analyzed for (D) white blood cell (WBC) count, (E) blood platelet count, (F) blood hemoglobin levels, (G) bone marrow cellularity, (H) percentage of LSK cells in the bone marrow, and (I) bone marrow colony-forming unit cells. The data in panels D–I are from n = 3–4 mice per group except in panels D-F in which the group size was n = 2 for Fancd2^−/−Fanci^−/− mice. P-values were determined by oneway Anova analysis (multiple comparisons) using a Prizm graphpad. Only statistically significant comparisons are shown (*P≤ 0.05, **P≤ 0.01, ***P≤ 0.001).