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. 2019 Jan 15;100(5):1132–1134. doi: 10.1093/biolre/ioz006

Figure 1.

Figure 1.

RNF216 is required for spermatogenesis and male fertility. (A), Western blotting showing expression levels of RNF216 in different mouse tissues. β-actin served as a loading control. (B), Western blotting showing expression levels of RNF216 at different stages of testis development. β-actin served as a loading control. (C), Rnf216 gene targeting strategy. Rnf216 mutant allele was generated by replacing exons 4 and 5 with a LacZ cassette. (D), Comparison of the body appearance of Rnf216+/− and Rnf216−/− mice. (E), Western blotting showing the absence of RNF216 protein in Rnf216−/− brain and testis. β-actin served as a loading control. (F), Gross morphology of adult Rnf216+/− and Rnf216−/− testes. (G), Testes weight of adult Rnf216+/− and Rnf216−/− mice. ***, P < 0.001; paired t-test. Error bars represent s.e.m. (H), Hematoxylin and eosin staining of adult Rnf216+/− and Rnf216−/− testes and epididymides. Upper, scale bar indicates 25 μm; lower, scale bar indicates 100 μm. (I), TUNEL assay of testis sections of 3-week-old Rnf216+/− and Rnf216−/−mice. Scale bar indicates 100 μm. (J-K), Fertility tests of male (J) and female (K) Rnf216+/− and Rnf216−/−mice. Error bars represent s.e.m.