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. 2019 May 31;47(14):7633–7647. doi: 10.1093/nar/gkz477

Figure 5.

Figure 5.

T. brucei small RNA interactome. (A) In vivo AMT-psoralen UV cross-linking was performed and used to identify interacting RNA molecules upon ligation. The RNA (-UV) and (+UV) was analyzed for intermolecular or intramolecular cross-links. The percentage of each of these species is presented. (B) Circos plot representing the interactome of snRNAs. The reads for these interactions are shown in (Supplementary Figure S8). (C) Validation of chimera between U2 snRNA and snoRNAs. (i) The ligation points of sn(o)RNA to U2 snRNA along its sequence following UV treatment are depicted on a linear scale; the control (-UV) is presented in (Supplementary Figure S9). Coordinates of the obtained chimera are given as the nucleotide on snoRNAs ligated to the position on U2 snRNA. (ii) Identification of snoRNAs that were affinity selected from cells subjected to AMT cross-linking, and affinity selection with U2 snRNA anti-sense oligonucleotide. The RPKMs of the different snoRNAs are presented as a bar diagram. The lines specify snoRNAs that were detected in both experiments used to identify the snoRNAs interacting with U2 snRNA.