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. 2019 May 25;47(14):7605–7617. doi: 10.1093/nar/gkz468

Figure 1.

Figure 1.

In vivo intron mobility assay. (A) Schematic of the assay showing intron donor plasmids (pGmS4S2095Fmal and pGmS4S2095), recipient lacZ gene and retrohoming product. LAG denotes that lagging strand DNA is used as primer for reverse transcription of the inserted intron RNA. In the lacZ gene the intron insertion site is shown (2095as). Psyn, syn promoter; EBS1,2,3, exon binding sites modified to recognize target 2095as into lacZ gene; E1, E2 short exons −20/+5; MF-IEP (MBP-Flag-IEP protein); IEP (wt IEP). (B) Comparison of retrohoming efficiencies between an intron expressing the wt IEP and the same intron expressing the MF-IEP fusion protein. Results are expressed as percentage of white colonies. Error bars are standard deviations (SDs) of three experiments.