Table 1.

Biophysical and pharmacological properties of currents recorded in CHO cells transfected with the indicated plasmid combinations

	cDNA transfected (µg)	n	V ½ (mV)	K (mV/efold)	Current density (pA/pF at 0 mV)	Blockade by 3 mmol/L TEA (%)
Nontransfected	‐	10	‐	‐	0.5 ± 0.1	‐
KCNQ3	3	9	−41.9 ± 1.5*, **	8.2 ± 0.9*, **	11.5 ± 4.8*, **	8.0 ± 2.1*, **
KCNQ3 p.(Phe534Ilefs*15)	3	9	‐	‐	0.3 ± 0.1	‐
KCNQ2 + pcDNA3	1.5 + 1.5	11	−21.7 ± 1.9	13.2 ± 0.8	21.7 ± 5.1*	‐
KCNQ2	3	13	−23.0 ± 1.5	12.0 ± 0.5	42.2 ± 9.7	94.0 ± 1.0**
KCNQ2 + KCNQ3	1.5 + 1.5	23	−35.1 ± 1.6	13.0 ± 0.7	117.6 ± 15.1	56.1 ± 6.6
KCNQ2  + KCNQ3 p.(Phe534Ilefs*15)	1.5 + 1.5	20	−23.9 ± 1.9**	15.4 ± 1.5	17.5 ± 2.5**	90.0 ± 1.5**
KCNQ2  +KCNQ3  +  pcDNA3	1.5 + 0.75 + 0.75	13	−27.5 ± 1.0**	13.0 ± 0.7	33.6 ± 6.9**	62.0 ± 4.3*
KCNQ2 + KCNQ3 + KCNQ3 p.(Phe534Ilefs*15)	1.5 + 0.75 + 0.75	24	−29.5 ± 1.8**	12.8 ± 0.7	39.6 ± 6.1**	62.2 ± 3.2*

^* P < 0.05 vs KCNQ2 (3 µg).

^** P < 0.05 vs KCNQ2 + KCNQ3 (1.5 + 1.5 µg).