NaCl (SLBS2340V) |
Sigma-Aldrich, St Louis, MO, USA |
NaHCO3 (SLBX3605) |
Sigma-Aldrich |
NaH2PO4 (BCBW9042) |
Sigma-Aldrich |
KCl (SLBS5003) |
Sigma-Aldrich |
MgCl2 (BCBS6841V) |
Sigma-Aldrich |
CaCl2 (SLBK1794V) |
Sigma-Aldrich |
Glucose (SLBT4811V) |
Sigma-Aldrich |
HEPES (W1122DO10) |
Sangon biological Shanghai, China |
2,3-butanedione monoxime (BDM) (29297) |
Sigma-Aldrich |
Blebbistatin (SLBV5564) |
Tocris Bioscience Minneapolis, MN, USA |
Voltage-sensitive dye RH237 (1971387) |
Thermo Fisher Scientific, Waltham, MA, USA |
Calcium indicator Rhod-2 AM (1890519) |
Invitrogen, Carlsbad, CA, USA |
Dimethyl sulfoxide (DMSO) (RNBT7442) |
Sigma-Aldrich |
Heparin Sodium (H51021209) |
Chengdu Haitong Pharmaceutical Co. Ltd. Chengdu, China |
Avertin (2,2,2-tribromoethanol) |
Sigma-Aldrich Poole, Dorset, UK |
Pluronic F127 (1899021) |
Invitrogen, Carlsbad, CA, USA |