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. 2019 Aug 8;10:879. doi: 10.3389/fphar.2019.00879

Figure 2.

Figure 2

Biological effect of the miR-21-specific miRNase. (A) Apoptotic profile of B16 melanoma cells after transfection with miR-21-miRNase and control luc-POC. Cytofluorimetric analysis of cells after staining with Annexin V-FITC/PI 48 h after transfection with miRNase or luc-POC (1 µM) in complex with Lipofectamine2000. Q1—population of living cells; Q2—Annexin V-FITC+/PI-, early apoptosis; Q3—Annexin V-FITC+/PI+, late apoptosis; Q4—Annexin V-FITC-/PI+, necrosis. (B) Average percentage of apoptotic (early and late apoptosis) and necrotic B16 cell population. Data represent mean ± s.e. of four independent experiments.(C, D) Invasion activity of B16 cells after incubation with Lipofectamine (LF) alone or treated with either hairpin antisense oligonucleotide (h-ODN), hairpin 2′-OMe oligonucleotide (2′-OMe), commercial miR-21 inhibitor (Inh), miR-21-miRNase, and control conjugate (luc-POC) in the concentration of 1 µM. Migration of cells was recorded in real time using the xCELLigence cell analysis system. Control—B16 cells without treatment. Data represent mean ± s.d. of four independent experiments.