Table 1.

The half-life time of miR-21 and reaction turnover in the presence of miR-21-miRNase and RNase H.

[miRNase], µM	[miR-21], µM	RNA excess	τ/21), h		Rt2)
			RNase H		RNase H
			–	+	–	+
5	10	2-fold	10.4 ± 0.5	0.7 ± 0.2	104 ± 10	1620±105
	25	5-fold	16.2 ± 1.5	5.4 ± 0.4	106 ± 11	740 ± 90
	50	10-fold	11.04 ± 0.8	4.7 ± 0.4	382 ± 28	1760±132

¹⁾τ/2—a half-life time of miR-21 in the presence of catalysts, the time during which 50% of miRNA molecules is cleaved.

²⁾Rt, reaction turnover—the number of miRNA molecules cleaved by one molecule of miRNase per 1 h. Rt was calculated according to equation Rt = [miR-21] × (number of bonds) × (cleavage, %)/[miRNase].