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. 2019 Aug 7;10:1827. doi: 10.3389/fmicb.2019.01827

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Copyright © 2019 Hirakawa, Suzue, Kurabayashi and Tomita.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fluorescent images of aggregated UPEC cells within bladder epithelial cells (HTB-9). Bacteria carrying a Green fluorescence protein (GFP) expression plasmid, pTurboGFP-B and HTB-9 stained with rhodamine- phalloidin were imaged as, respectively, green and red fluorescent colors on the microscopy using 60× objective. Overlooking images (A) and cross sectional images in the white box indicated in panel A (B) were acquired for each sample. The experiment was repeated twice and similar results were obtained.