FIGURE 5.

Motilities of the wild-type parent and tolB mutant, or wild-type parent and tolB mutant carrying pTrc99K (empty vector) or pTrc99KtolB (tolB expression plasmid). (A) Bacterial migrations on RPMI1640 medium containing 0.3% agar after 10 h at 37°C under 5% of CO₂ were pictured. (B) Bacterial migrations on the agar were represented as diameters. Data plotted are the means from three independent experiments; error bars indicate the standard deviations ^*P < 0.01. Asterisks denote significance for values relative to the wild-type control. (C) Flagella and bacteria cells stained with Victoria blue/Tannic acid were pictured on the microscopy using 100× objective. (D) Transcript levels of motility-related genes in the wild-type parent and the tolB mutant. Transcript levels were described as relative values to that of rpoD (housekeeping gene). Data plotted are the means of two biological replicates, error bars indicate the ranges.