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. 2019 Aug 8;9:743. doi: 10.3389/fonc.2019.00743

Figure 4.

Figure 4

Carnosol inhibits the STAT3 signaling pathway. (A) Concentration-dependent decrease of phospho-STAT3 and STAT3 protein in carnosol-treated MDA-MB-231 cells. Cells were treated with vehicle (DMSO) or indicated concentrations of carnosol for 24 h, then whole-cell extracts were subjected to Western blot analysis for the phosphorylated and non-phosphorylated form of STAT3 and for β-actin (loading control). (B) Carnosol decreases the level of STAT3 protein in Hs578T, MCF-7, and T47D breast cancer cell lines. Whole cell lysates from different breast cancer cell lines treated with the indicated concentrations of carnosol were resolved on 8% SDS-PAGE and analyzed by western blot for STAT3 protein. (C) Western blot analysis of STAT3 protein level in HCT116 colorectal cancer cells. Western blotting was performed as described above.