Figure 7.

Anti-tumor growth and anti-metastatic activity of carnosol on breast tumor in chick embryo chorioallantoic membrane assay (A) MDA-MB-231 (1 ×10⁶) cells were grafted on the CAM of 10 day (E10) chick embryo. Tumors were treated every 48 h with carnosol (50 and 100 μM) as described in Materials and Methods. At E19, tumors were collected and weighted. (B) Quantification of tumor weight in vehicle-, colchicine- and carnosol-treated chick embryo. (C) Anti-metastatic effect of carnosol. Quantification of nodules observed in the lower CAM of chick embryo treated with vehicle, colchicine or indicated concentrations of carnosol. Columns represents mean; bars represent SEM (***p < 0.001).