Table 4.

Summary of the performance of 16S rRNA, sodA, rpoB, tuf genes and 16S-23S rRNA region sequencing used for differentiation of Staphylococcus genus.

	16S rRNA gene	sodA gene	tuf gene	rpoB gene	NGS 16S-23S rRNA
Unambiguous species identification	27 species (54%)	48 species (96%)	41 species (82%)	48 species (96%)	45 species (90%)
The lowest amount of nucleotides differences	0	0	0	0	0
The highest amount of nucleotides differences	57	149	120	192	667
No. of species without reference sequences in the databases	0	7	9	8	21