. 2019 Aug 7;10:1837. doi: 10.3389/fmicb.2019.01837

TABLE 2.

Effect of trans-cinnamaldehyde (TC; 18.91, 37.82, 75.64 mM), eugenol (EG; 15.22, 30.45, 60.90 mM), and carvacrol (CR; 16.64, 33.28, 66.56 mM) on mature Campylobacter jejuni NCTC 11168 biofilm formed on polystyrene microtiter plates at 20°C or 37°C in the broth medium and in the presence of chicken meat juice (n = 6).

		20°C						37°C
		Broth medium			Broth + 5% chicken juice			Broth medium			Broth + 5% chicken juice
Treatments		1 min	5 min	10 min	1 min	5 min	10 min	1 min	5 min	10 min	1 min	5 min	10 min
Control		8.10 ± 0.10^a*	7.79 ± 0.08^a	7.78 ± 0.08^a	7.81 ± 0.05^a	7.81 ± 0.05^a	7.81 ± 0.05^a	8.35 ± 0.11^a	8.44 ± 0.10^a	8.39 ± 0.09^a	8.59 ± 0.04^a	8.66 ± 0.03^a	8.63 ± 0.04^a

TC (mM)	18.91	6.80 ± 0.09^b	5.63 ± 0.08^b	4.54 ± 0.07^b	6.69 ± 0.11^b	6.15 ± 0.04^b	5.14 ± 0.04^b	7.80 ± 0.05^b	7.78 ± 0.05^b	7.54 ± 0.04^b	8.38 ± 0.06^ab	8.26 ± 0.03^b	7.98 ± 0.04^b
	37.82	6.41 ± 0.07^c	4.32 ± 0.09^c	4.00 ± 0.06^cd	6.45 ± 0.14^b	5.19 ± 0.06^c	4.89 ± 0.03^bc	7.62 ± 0.05^bc	7.53 ± 0.04^b	7.39 ± 0.07^b	7.67 ± 0.04^d	7.68 ± 0.06^c	7.60 ± 0.14^c
	75.64	ND^e	ND^d	ND^e	5.27 ± 0.06^cd	4.89 ± 0.06^c	4.59 ± 0.07^c	7.68 ± 0.12^b	5.79 ± 0.09^d	5.61 ± 0.07^d	7.52 ± 0.03^d	7.36 ± 0.05^c	6.99 ± 0.19^d

EG (mM)	15.22	6.58 ± 0.05^bc	5.40 ± 0.09^b	4.52 ± 0.06^b	6.68 ± 0.09^b	6.20 ± 0.05^b	5.00 ± 0.07^bc	7.49 ± 0.06^bc	6.47 ± 0.49^c	6.34 ± 0.10^c	8.09 ± 0.08^bc	7.66 ± 0.16^c	6.75 ± 0.16^de
	30.45	6.09 ± 0.07^d	4.34 ± 0.05^c	3.91 ± 0.09^d	6.38 ± 0.04^b	5.11 ± 0.05^c	4.82 ± 0.02^c	7.57 ± 0.11^bc	5.59 ± 0.10^d	4.95 ± 0.16^e	7.84 ± 0.25^cd	6.16 ± 0.04^e	5.72 ± 0.07^f
	60.90	ND^e	ND^d	ND^e	5.01 ± 0.06^de	4.89 ± 0.03^c	3.93 ± 0.08^d	7.31 ± 0.08^c	5.16 ± 0.21^e	4.55 ± 0.27^fg	6.67 ± 0.38^e	5.18 ± 0.11^f	4.52 ± 0.20^g

CR (mM)	16.64	6.37 ± 0.07^cd	5.25 ± 0.11^b	4.33 ± 0.07^bc	5.44 ± 0.01^c	5.16 ± 0.04^c	4.80 ± 0.08^c	6.94 ± 0.17^d	6.30 ± 0.11^c	6.07 ± 0.05^c	6.91 ± 0.16^e	6.76 ± 0.05^d	6.46 ± 0.09^e
	33.28	6.14 ± 0.07^cd	4.26 ± 0.09^c	3.98 ± 0.07^cd	4.73 ± 0.09^e	4.49 ± 0.05^d	4.03 ± 0.11^d	5.87 ± 0.15^e	5.55 ± 0.05^d	4.89 ± 0.09^ef	5.41 ± 0.12^f	5.18 ± 0.05^f	4.50 ± 0.09^g
	66.56	ND^e	ND^d	ND^e	3.30 ± 0.32^f	3.11 ± 0.07^e	1.12 ± 0.26^e	5.76 ± 0.09^e	5.07 ± 0.25^e	4.46 ± 0.09^g	4.90 ± 0.07^g	4.77 ± 0.13^g	3.75 ± 0.13^h

The biofilms were exposed to phytochemical treatment for 1, 5, or 10 min. Values (Log CFU/mL) presented as mean ± standard error of the mean. ^*The different superscripts within columns differ significantly at P < 0.05. ND: not detectable below 1 Log CFU/mL.