Table 2.
Primers of CAT, Mn-SOD, GPx, and β-actin for real-time PCR1.
| Objective gene |
Accession number |
Primer sequence (5′ to 3′) | Annealing temperature (°C) | Amplicon length |
|---|---|---|---|---|
| CAT | NM_001031215.2 | Forward: CTTTCCCTCTTCCCTTACCA | 58.24 | 182 |
| Reverse: GGTAGTAGCTGGGCTCTGAAA | 58.62 | |||
| Mn-SOD | AF299388.1 | Forward: ACAGATAGCAGCCTGTGCAA | 59.62 | 163 |
| Reverse: CGCGTTCTCCCAGTTGAT | 59.79 | |||
| GPX | U22046.1 | Forward: GACGGCTGAGAGTTGATCCT | 59.41 | 176 |
| Reverse: CTGCGGGTATTTGATGTCC | 58.94 | |||
| β-actin | NM_205518.1 | Forward: AGTTACTCGCCTCTGTGAAGG | 94.59 | 198 |
| Reverse: CACATCTATCACTGGGGAACAC | 33.59 |
1CAT: Catalase; Mn-SOD: Manganese super oxide dismutase; GPX: Glutathione peroxide1; β-actin: Internal control.