Table 1.
Description of available anti-CD146 antibodies.
| Antibody | Utilization | Antibody generation |
|---|---|---|
| MUC 18 | IHC, WB | Mouse immunization with a cell lysate of metastazing melanoma (Lehmann JM et al., Cancer Research, 1987) [1]. |
| S-endo 1 (Biocytex) | IF, IHC, WB, FC | Mouse immunization with a HUVEC lysate (George et al., J. Immunol. Methods, 1991) [7]. |
| 7A4 (Biocytex) | IF, WB, FC, ELISA, IP | Mouse immunization with an injection of CD146 |
| AA98 | Bloquing in vivo, WB, IHC, IF, WB | Mouse immunization with a lysate of HUVEC stimulated with conditioned medium of hepatoma cell line SMMC 7721 (Yan et al., Blood, 2003 ) [9]. |
| ABX-MA1 | WB, FC, IHC | Generated using Abgenix’s proprietary XenoMouse mice (Mills et al,, Cancer Res., 2002) [8]. |
| M2J-1 | Bloquing in vivo, ELISA | Rat immunization with an injection of recombinant soluble CD146 (Stalin et al., Oncogene, 2016) [4]. |
| TsCD146 | IF, FC, WB, PET/SPECT-CT, bloquing in vivo | Rat immunization with an injection of recombinant soluble CD146 (Nollet et al., in process) [10]. |
| YY146 | PET/SPECT-CT | Mouse immunization with an injection of the human CD146 antigen (Yang et al., 2016, Proc Natl Acad Sci) [14]. |
| OI-3 | Radiolabeling, FC | Mouse immunization with an injection of recombinant chimeric versions of human IgG1 or human IgG3 Fc sequences (Westrøm et al, 2016, PLoS One) [11]. |
| 5G6 (Biocytex) | IF | Mouse immunization with an injection of immunopurified CD146 |
| 2F6 (Biocytex) | IF | Mouse immunization with an injection of immunopurified CD146 |
| 3D9 (Biocytex) | IF, FC | Mouse immunization with an injection of immunopurified CD146 |
| Antibody against short or long isoforms of CD146 | WB, IF, FC, IP | Rabbit immunization with an injection of peptides corresponding to the intracellular part of short and long CD146 (Kebir et al., Circulation research, 2010) [3]. |
| P1H12 (Abcam) | IF, IHC, WB, FC, IP, ELISA | Mouse immunization with a HUVEC lysate (Solovey et al., J Lab Clin Med, 2001) [56] |
| EPR3208 (Abcam) | IF, IHC, FC, WB | Rabbit immunization with an injection of a synthetic peptide corresponding to Human CD146 AA 600 to the C-terminus |
| 541-10B2 (Miltenyi Biotec) | FC | Mouse immunization |
| AA1 | WB | Mouse immunization with CD146 purified from HUVEC (Zhang et al., Hybridoma, 2008) [63] |
In this table are listed the different anti-CD146 antibodies, their characteristics and how they were generated. IHC = immunohistochemistry, WB = Western blot, IF = immunofluorescence, FC = flow cytometry, IP = immuno-precipitation, PET = positron emission tomography, ELISA = enzyme-linked immunosorbent assay, SPECT-CT = single-photon emission tomography / computed tomography.