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. 2016 Mar 23;5(2):7. doi: 10.3390/antib5020007

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© 2016 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Microscopic analysis of surface staining of sialoadhesin (Sn) on CHO cells. Monoclonal antibodies (mAbs) were added to fixed Sn⁺ CHO cells to analyze the recognition of surface Sn. Secondary Alexa Fluor 488-labeled antibodies were added to bind with the mAbs (green), nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Wild-type CHO cells were used as control cells. (A) All anti-hSn mAbs were tested against hSn⁺-CHO and pSn⁺-CHO; (B) anti-mSn mAbs were used to stain mSn⁺-CHO; (C) reference mAbs 7D2, 3D6, and 41D3 were analyzed for recognition of hSn⁺-, pSn⁺-, and mSn⁺-CHO cells, respectively.