Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Mar 23;5(2):7. doi: 10.3390/antib5020007

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Flow cytometric analysis of internalization capacity of different mAbs on Sn⁺-CHO cells. The cells were incubated with different mAbs to allow only binding to surface-expressed (A) hSn; (B) pSn; and (C) mSn. Afterwards the cells were shifted to 37 °C during 60 min to induce internalization of Sn. Secondary fluorescently-labeled antibodies were added to the cells, staining only remaining surface-Sn. Only viable cells were analyzed with flow cytometry using PI staining. Data represent the mean and standard error of the mean of three independent repeats. Relative internalization was calculated by comparing the fluorescent signal to that of cells without internalization.