Table 3.
Stability of Concentrated Proteins Stored at 4 °C (concentration of antibodies over time).
| Sample ID | Release concentration (mg/mL) | Week 1 (mg/mL) | Week 2 (mg/mL) | Week 3 (mg/mL) | Week 4 (mg/mL) |
|---|---|---|---|---|---|
| IgG1 | 53.9 ± 0.8 | 53.1 ± 0.3 | 57.2 ± 0.7 | 55.1 ± 0.4 | 55.0 ± 0.3 |
| IgG1 FES | 45.5 ± 0.9 | 48.8 ± 1.2 | 46.4 ± 0.7 | 46.7 ± 0.3 | 46.6 ± 1.5 |
| IgG1σ | 41.9 ± 0.6 | 41.5 ± 0.2 | 42.0 ± 0.3 | 42.5 ± 0.9 | 43.4 ± 0.5 |
| IgG2 | 41.4 ± 0.1 | 40.5 ± 0.2 | 42.0 ± 0.4 | 42.9 ± 0.2 | 43.0 ± 0.8 |
| IgG2σ | 40.8 ± 0.5 | 41.3 ± 0.4 | 40.9 ± 0.6 | 41.7 ± 1.1 | 41.8 ± 0.6 |
| IgG4-PAA | 48.6 ± 1.5 | 50.6 ± 0.6 | 52.5 ± 1.0 | 53.2 ± 0.2 | 50.9 ± 1.3 |
| IgG4σ1 | 50.4 ± 0.4 | 50.3 ± 0.1 | 50.0 ± 0.6 | 50.0 ± 0.2 | 51.4 ± 0.8 |
| IgG4σ2 | 51.7 ± 1.1 | 51.3 ± 0.4 | 48.4 ± 2.1 | 53.4 ± 1.6 | 54.8 ± 0.7 |
Ab samples are in PBS and shown as mean ± range. Abbreviations: IgG1 AA, IgG1 L234A/L235A; IgG1 FES, IgG1 L234F/L235E/P331S; IgG4 PAA, IgG4 S228P/F234A/L235A.