Figure 1.

Binding of human and animal IgGs to Zn-beads. (a) Aliquots (1 mL) of IgG (25 µg) and Zn-beads (Zn) or CB in phosphate-buffered saline (PBS) were prepared (net volume of beads per sample: 20 µL each) and incubated at 4 °C overnight. The mixture was centrifuged at 14,000× g for 7 min and the supernatant (S) and pelleted beads (B) were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) as described in the “Experimental Section”. Human IgG (IgG) was also applied to the gel (2 µg/lane) and the separated H and L subunits are denoted H and L, respectively. M represents marker proteins; (b) Animal IgG samples were treated as described in (a), and only the resultant beads were analyzed by SDS-PAGE. The H and L subunits from the various animal IgGs (2 µg each per lane) were denoted H and L. M represents marker proteins.