Figure 3.

The binding of human IgG Fab (a) and F(c) fragments (b) to Zn-beads. Aliquots (1 mL) of each fragment (25 µg) and Zn-beads or CB in PBS were prepared (net volume of beads per sample: 20 µL each), and incubated at 4 °C overnight. The mixtures were centrifuged at 14,000× g for 7 min, and the supernatant and pelleted beads were subjected to SDS-PAGE as described in the “Experimental Section”. Supernatant (S) and pelleted beads (B) from respective CB and Zn-beads (Zn) incubated with Fab (a) and F(c) (b) were applied to SDS-PSGE. Human IgG Fab and F(c) fragment samples were also applied to the gel (2 µg each/lane). M represents marker proteins.