Figure 1.

Selection of conformation-specific single-chain variable domain (scFv)-phage. The process of the panning started with depleting the scFv-phage library on non-activated cells having unwanted and non-stimulated epitopes. The non-specific scFv-phage/non-activated cell complex were centrifuged and discarded, while the supernatant containing the free scFv-phage were transferred into physiologically stimulated cells expressing neo-epitopes in their active conformational structure. The scFv-phage/activated cell complexes were centrifuged and the bound scFv-phage were both eluted with low pH or by competition with ligand, and collected for the subsequent panning rounds. Figure shows methodology described by Eisenhardt et al. [94].