Figure 4.

Whole cell-based panning using Biopanning and Rapid Analysis of Selective Interactive Ligands (BRASIL). Panning started with a depletion step for the phage library on cells not expressing the antigen of interest. The phage/unwanted cells complex were centrifuged into the organic phase. Free phage within the aqueous supernatant are transferred and incubated with cells expressing the antigen of interest into the aqueous solution before centrifugation through the organic phase. Bound phage were rescued from the organic phase, and then amplified to be used for the subsequent panning rounds. Figure shows methodology described by Giordano et al. [126].