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. 2017 Oct 15;6(4):14. doi: 10.3390/antib6040014

Figure 1.

Figure 1

Characterization of nonstructural protein 1-specific (NS1) monoclonal antibodies (mAbs) reactivity by enzyme-linked immunosorbent assay (ELISA). Reactivity of mAbs to heated-treated or intact rNS1, as solid phase-bound antigens. The mAbs 4F6 (A), 4H2 (B), 2H5 (C) and 4H1BC (D) were serially diluted (log2) from an initial concentration of 2.5 µg/mL. Each well was adsorbed with 400 ng of rNS1. Heat denaturation was performed at 100 °C for 10 min. Statistical analyses were performed by two-way variance analysis followed by Bonferroni’s post-test. (*** p < 0.01; ** p < 0.1; * p < 0.5).