Table 1.
Characteristics of the monoclonal antibodies (mAbs) against dengue virus (DENV) nonstructural protein 1 (NS1).
| Name | 4F6 | 4H2 | 2H5 | 4H1BC |
|---|---|---|---|---|
| IgG Subtype a | IgG2a | IgG2a | IgG1 | IgG1 |
| DENV2 NS1 reactivity b | Yes | Yes | Yes | Yes |
| Dissociation Constant (KD) c | 1.1 × 10−8 M | 6.2 × 10−8 M | 7.3 × 10−8 M | 8.4 × 10−8 M |
| Detection limit d | 16 ng/mL | 32 ng/mL | 32 ng/mL | 32 ng/mL |
| Epitope sequence e | 25VHTWTEQYKFQPES38 | 127ELHNQTFLIDGPETAEC143 | 193AVHADMGYWIESALNDT209 | 193AVHADMGYWIESALNDT209 |
| DENV (1–4) reactivity f | No | Yes | No | No |
| ZIKV reactivity g | No | No | Yes | Yes |
a The Ig isotype and IgG subtypes were performed by enzyme-linked immunosorbent assay (ELISA) using anti-IgA, anti-IgM, anti-IgG1, anti-IgG2a, anti-IgG2b and anti-IgG3 coated onto microplates; b The Dengue virus serotype 2 (DENV2) NS1 reactivity was evaluated by indirect ELISA and immunoblotting using rNS1; c Dissociation constant was performed by ELISA [18]; d Detection limit was evaluated by ELISA using different concentrations of rNS1; e The conservancy of DENV2 NS1 epitopes recognized by specific mAbs in a peptide array was analyzed among the four serotypes of DENV, using three samples of NS1 amino acid sequences as representative of each DENV serotype; f,g DENV (1–4) and Zika virus (ZIKV) reactivity was evaluated by immunofluorescence in Vero cells infected with the specific virus strains.