Table 6.
Classification of CHO proteins identified via LC-MS/MS analysis and characterized by 2D-PAGE gel. Comparison of theoretical (UniProt; pI calculated according to the amino acid sequences) and observed MW and pI with respect to the spot position on the 2D-PAGE gel.
| Spot Gel | MW Gel | pI Gel | Class Gel | MW 1 | pI 2 | Class MS | Protein | UniProt Accession Number |
|---|---|---|---|---|---|---|---|---|
| 1 | 25 | 7.0 | Bad | 81.56 | 5.69 | Bad | Glutathione S-transferase Mu7-like protein |
A0A061IN16 |
| 2 | 25 | 7.5 | Ugly | 102.7 | 6.02 | Bad | Actin, cytoplasmic 1 | A0A069C7Y3 |
| 3 | 30 | 7.6 | Ugly | 38.03 | 6.08 | Bad | Purine nucleoside phosphorylase-like protein |
A0A061ILE8 |
| 4 | 50 | 9.4 | Bad | 72.13 | 7.23 | Ugly | Pyruvate kinase | A0A098KXF7 |
| 5 | 25 | 6.3 | Bad | 38.03 | 6.08 | Bad | Purine nucleoside phosphorylase-like protein |
A0A061ILE8 |
1 Theoretical values according to the unmodified full length protein according to UniProt; 2 Theoretical values calculated using the ExPASy computation tool (http://web.expasy.org/compute_pi/).