Table A2.
Peptides and their corresponding proteins identified via LC-MS/MS in spots of 2D gel of the diafiltrated broth. Molecular weight (MW) and isoelectric point (pI) of the unmodified full length protein according to UniProt.
| # | Gel MW | Gel pI | MW (UniProt) | pI (UniProt) | Primary accession number (UniProt) | Number of Unique Peptides | Protein |
|---|---|---|---|---|---|---|---|
| 1 | 25 | 7 | 26.96 | 5.38 | A0A061I6A0 | 5 | Glutathione S-transferase A4-like protein |
| 81.56 | 5.69 | A0A061IN16 | 8 | Glutathione S-transferase Mu 7-like protein | |||
| 38.03 | 6.08 | A0A061ILE8 | 8 | Purine nucleoside phosphorylase-like protein | |||
| 2 | 25 | 7.5 | 38.03 | 6.08 | A0A061ILE8 | 6 | Purine nucleoside phosphorylase |
| 102.7 | 6.02 | A0A069C7Y3 | 2 | Actin, cytoplasmic 1 | |||
| 43.35 | 6.48 | A0A061IJG8 | 2 | Prostaglandin reductase 1-like protein | |||
| 3 | 30 | 7.4 | 45.28 | 8.48 | A0A061IB69 | 1 | Fructose-bisphosphate aldolase |
| 4 | 50 | 9.4 | 89.52 | 6.23 | A0A061IJC4 | 2 | Glutathione S-transferase |
| 5 | 25 | 6.6 | 38.03 | 6.08 | A0A061ILE8 | 7 | Purine nucleoside phosphorylase |
| 89.52 | 6.23 | A0A061IJC4 | 5 | Glutathione S-transferase | |||
| 6 | 37 | 9.4 | 45.28 | 8.48 | A0A061IB69 | 4 | Fructose-bisphosphate aldolase |
| 43.35 | 6.48 | A0A061IJG8 | 2 | Prostaglandin reductase 1-like protein | |||
| 361.89 | 4.81 | A0A061IH02 | 2 | Desmoglein-4-like protein | |||
| 7 | 30 | 7 | 38.03 | 6.08 | A0A061ILE8 | 4 | Purine nucleoside phosphorylase |
| 128.68 | 6.78 | A0A061IK77 | 3 | Exosome component 10 isoform 1 | |||
| 11.37 | 11.36 | G3H2T6 | 2 | Histone H4 | |||
| 8 | 30 | 6.8 | 27.79 | 4.7 | A0A061IGS6 | 4 | Protein sigma |
| 102.7 | 6.02 | A0A069C7Y3 | 5 | Actin, cytoplasmic 1 | |||
| 361.89 | 4.81 | A0A061IH02 | 4 | Desmoglein-4-like protein | |||
| 9 | 17 | 6.6 | 11.37 | 11.36 | G3H2T6 | 4 | Histone H4 |
| 14.99 | 10.2 | A0A061IP52 | 2 | Histone H2B | |||
| 10 | 30 | 5.6 | 52.25 | 5.35 | A0A061IML2 | 13 | Annexin |
| 268.7 | 5.69 | A0A061IP39 | 10 | Filamin-B isoform 4 | |||
| 50.99 | 6.94 | A0A061I8I4 | 4 | Cathepsin F | |||
| 11 | 30 | 4.5 | 52.25 | 5.35 | A0A061IML2 | 2 | Annexin |
| 14.73 | 9.87 | A0A061IQB8 | 3 | Ubiquitin-60S | |||
| 12 | 30 | 2.8 | 38.03 | 6.08 | A0A061ILE8 | 8 | Purine nucleoside phosphorylase |
| 89.52 | 6.23 | A0A061IJC4 | 8 | Glutathione S-transferase | |||
| 101.51 | 5.12 | A0A061IRD9 | 5 | AP complex subunit beta | |||
| 13 | 15 | 6.7 | 38.03 | 6.08 | A0A061ILE8 | 4 | Purine nucleoside phosphorylase |
| 89.52 | 6.23 | A0A061IJC4 | 4 | Glutathione S-transferase | |||
| 38.31 | 5.33 | A0A061IEW1 | 3 | Nuclear migration protein nudC-like protein | |||
| 14 | 15 | 6.1 | 38.31 | 5.33 | A0A061IEW1 | 5 | Nuclear migration protein nudC-like protein |
| 89.52 | 6.23 | A0A061IJC4 | 4 | Glutathione S-transferase | |||
| 54.11 | 5.01 | A0A061IDB2 | 3 | Prelamin-A/C-like isoform 1 | |||
| 15 | 12 | 5.6 | 17.16 | 7.8 | A0A061I0I3 | 4 | SH3 binding protein |
| 17.19 | 5.94 | G3HBD4 | 3 | Nucleoside diphosphate kinase | |||
| 16 | 17 | 6.6 | 23.42 | 5.1 | G3GXB0 | 3 | Rho GDP |
| 89.52 | 6.23 | A0A061IJC4 | 8 | Glutathione S-transferase | |||
| 102.7 | 6.02 | A0A069C7Y3 | 2 | Actin, cytoplasmic 1 | |||
| 17 | 16 | 9.2 | 14.73 | 9.87 | A0A061IQB8 | 3 | Ubiquitin-60S |
| 102.7 | 6.02 | A0A069C7Y3 | 3 | Actin, cytoplasmic 1 | |||
| 23.42 | 5.1 | G3GXB0 | 3 | Rho GDP |