Table A3.
Peptides and their corresponding proteins identified via LC-MS/MS in spots of 2D gel of the HIC fraction. Molecular weight (MW) and isoelectric point (pI) of the unmodified full length protein according to UniProt.
| # | Gel MW | Gel pI | MW (UniProt) | pI (UniProt) | Primary Accession Number (UniProt) | Number of Unique Peptides | Protein |
|---|---|---|---|---|---|---|---|
| 1 | 25 | 6.9 | - | - | - | - | - |
| 2 | 25 | 7.6 | 11.37 | 11.36 | G3H2T6 | 2 | Histone H4 |
| 102.7 | 6.02 | A0A069C7Y3 | 2 | Actin, cytoplasmic 1 | |||
| 3 | 30 | 7.6 | 38.03 | 6.08 | A0A061ILE8 | 2 | Purine nucleoside |
| 102.7 | 6.02 | A0A069C7Y3 | 1 | Actin, cytoplasmic 1 | |||
| 4 | 50 | 8.4 | 44.67 | 7.54 | A0A061IJI8 | 8 | Alpha-enolase |
| 72.13 | 7.23 | A0A098KXF7 | 8 | Pyruvate kinase | |||
| 38.03 | 6.08 | A0A061ILE8 | 2 | Purine nucleoside | |||
| 5 | 25 | 6.3 | 38.03 | 6.08 | A0A061ILE8 | 6 | Purine nucleoside |
| 6 | 25 | 8.4 | 38.03 | 6.08 | A0A061ILE8 | 4 | Purine nucleoside |
| 7 | 50 | 8 | 44.67 | 7.54 | A0A061IJI8 | 1 | Alpha-enolase |
| 8 | 47 | 7.6 | 44.67 | 7.54 | A0A061IJI8 | 5 | Alpha-enolase |
| 102.7 | 6.02 | A0A069C7Y3 | 3 | Actin, cytoplasmic 1 | |||
| 72.13 | 7.23 | A0A098KXF7 | 2 | Pyruvate kinase | |||
| 9 | 50 | 7.2 | 59.76 | 9.22 | A0A061ICE4 | 4 | ATP synthase subunit |
| 14.73 | 9.87 | A0A061IQB8 | 2 | Ubiquitin-60S ribosomal protein L40-like isoform 2 | |||
| 102.7 | 6.02 | A0A069C7Y3 | 2 | Actin, cytoplasmic 1 | |||
| 10 | 25 | 5.1 | - | - | - | - | - |
| 11 | 50 | 4.8 | 211.66 | 5.42 | A0A061I4N6 | 1 | CAP-Gly domain-containing linker protein 1 |
| 12 | 52 | 4 | - | - | - | - | - |
(-) Spots, which were not able to be identified.