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. 2019 Jul 11;105(2):403–412. doi: 10.1016/j.ajhg.2019.06.007

Figure 5.

Figure 5

Bioluminescence Resonance Energy Transfer Assays

(A) Bioluminescence resonance energy transfer (BRET) assays to measure interactions between WT POU3F3 and mutant POU3F3 constructs. Bars represent the corrected mean BRET ratio ± SD of three independent experiments performed in triplicate (∗∗∗∗ = p < 0.0001; ∗ = p < 0.05; and NS = not significant when compared to WT using one-way ANOVA and a post-hoc Tukey’s test). The NLS-donor construct is a Renilla luciferase construct with a nuclear localization signal.

(B) BRET assays to measure homodimerization capacity of each mutant POU3F3 construct. Bars represent the corrected mean BRET ratio ± SD of three independent experiments performed in triplicate (∗∗∗∗ = p < 0.0001; ∗∗∗ = p < 0.001; and NS = not significant when compared to WT using one-way ANOVA and a post-hoc Tukey’s test)