KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse anti-EB1 | BD Transduction | Cat#: 610535 |
| Rat anti-EB3 | Absea | Cat#: 010314H04 |
| Rabbit anti-Giantin | Abcam | Cat#: ab24586 |
| Mouse anti-GM130 | BD Transduction | Cat#:610823 |
| Rabbit EPAC1 | Abcam | Cat#: ab124162 |
| Rabbit EPAC2 | Abcam | Cat#: ab124189 |
| Mouse GAPDH | Santa Cruz | Cat#: sc-32233 |
| Mouse anti-E cadherin | BD Transduction | Cat#: 610181 |
| Guinea Pig anti-GCC185 | [44] | N/A |
| Guinea Pig anti-Insulin | Agilent Technologies | Cat#: A0564 |
| Rabbit anti-β-tubulin | Abcam | Cat#: ab18251 |
| Anti-GFP FITC | Abcam | Cat#: ab6662 |
| Chemicals | ||
| Vectashield Mounting Medium | Vector Labs | Cat#: H-1000 |
| Nocodazole | Sigma-Aldrich | Cat#: M1404 |
| Verapamil hydrochloride | MP Biomedicals | Cat#: 0219554501 |
| MPA | Sigma-Aldrich | Cat#: M5255 |
| GKA50 | Sigma-Aldrich | Cat#: SML0849 |
| Diazoxide | Tocris Bioscience | Cat#: 0934 |
| Metformin | Sigma-Aldrich | Cat#: PHR1084 |
| Sodium Pyruvate | ThermoFisher | Cat#: 11360070 |
| KCl | EM Science | Cat#: PX1405-1 |
| 8-br-cAMP | Sigma-Aldrich | Cat#: B7880 |
| 8-br-cAMP-AM | Biolog | Cat#: B 020 |
| Ionomycin | Sigma-Aldrich | Cat#: I3909 |
| PKI | Sigma-Aldrich | Cat#: P9115 |
| Forskolin | Sigma-Aldrich | Cat#: F6886 |
| 8-pCPT-2O-Me-cAMP | Sigma-Aldrich | Cat#: C8988 |
| HJC0197 | Cayman Chemical | Cat#: 19092 |
| ESI-09 | Sigma-Aldrich | Cat#: SML0814 |
| BAPTA-AM | Invitrogen | Cat#: B1205 |
| D (+)-glucose | Acros | Cat# 41095-0010 |
| NaHCO3 | HyClone | Cat# SH30173.04 |
| EGTA | Sigma-Aldrich | Cat# E3889 |
| NaCl | Sigma-Aldrich | Cat# S9625 |
| MgSO4 | Sigma-Aldrich | Cat# M7506 |
| KH2PO4 | ThermoFisher | Cat# BP363 |
| HEPES | Sigma-Aldrich | Cat# H4034 |
| CaCl2 | Sigma-Aldrich | Cat# C1016 |
| BSA | ThermoFisher | Cat# BP1605 |
| Critical Commercial Assays | ||
| Mouse Ultrasensitive Insulin ELISA | Alpco | 80-INSMSU-E10 |
| Experimental Models: Cell Lines | ||
| MIN6 cells | [52] | N/A |
| hTERT RPE-1 cells | ATCC | Cat#: CRL-4000 |
| Experimental Models: Organisms/Strains | ||
| CD-1 (ICR) Mice | Charles River Inc. | Stain code:022 |
| Oligonucleotides | ||
| Cdk5Rap2 51-100 Fwd | Sigma-Aldrich | GGCTCGAGGCCGCGAATTCCACAGT |
| Cdk5Rap2 51-100 Rev | Sigma-Aldrich | GGATGCCACCCCGGGATCCTC |
| shRNA | ||
| EPAC2 Mouse shRNA #1 | Origene | TL509420A CGACAAGGAAGACTTCAATCGGATTCTGA |
| EPAC2 Mouse shRNA #2 | Origene | TL50942C CCATTACCACGCACAGCCTTCTCAAGGTA |
| Recombinant DNA | ||
| pCMV Cdk5Rap2 51-100 F75A | [42] | N/A |
| pCIG | McMahon Plasmid Database | 2165 |
| Emerald-EB3 | Addgene | Plasmid# 54076 |
| TGN-RFP | [53] | N/A |
| Dominant negative AKAP450 | [41] | N/A |
| Software and Algorithms | ||
| Graphpad Prism | Graphpad | https://www.graphpad.com |
| Image J | Image J | https://imagej.nih.gov/ij/ |
| Adobe illustrator/photoshop | Adobe | http://www.adobe.com |
| Imaris | Bitplane | http://www.bitplane.com |