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. 2018 Nov 7;7(4):39. doi: 10.3390/antib7040039

Figure 6.

Figure 6

SsZntA-Nb complex analysis using size-exclusion chromatography. (a) Black line: Chromatogram of SsZntA (40 µM) incubated with Nb1 (100 µM) in a final volume of 50 µL (then diluted to 500 µM) and run through a size-exclusion column (Superose6, 1 CV = 24 mL) showing a peak at 0.6 CV, which contains the SsZntA-Nb complex, and a peak at 0.85 CV, which contains unbound Nb. The dashed line represents the chromatogram of Nb1 alone, while the dotted line the one from SsZntA alone (under the same condition). (b) Coomassie-stained SDS-PAGE gel of the fractions collected at 0.35, 0.5, 0.55, 0.6, 0.65, 0.8, 0.85, 0.9, 1 CV.