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SsZntA-Nb complex analysis using affinity chromatography: SsZntA + Nb. (a) Chromatogram of HisTag-free SsZntA incubated with HisTagged Nb1. The complex elutes at 400 mM imidazole and is here reported as representative of the other Nbs. (b) Coomassie (top) and InVision® (bottom) stained SDS-PAGE of the eluted fractions at 400 mM imidazole of HisTag-free SsZntA in complex with, respectively, Nb1-2-4-5-8-9.
