Figure 2.

L‐glutamine treatment upregulated HSP70, increased antioxidant levels, and reduced inflammatory response in stroke mice. A, Western blot analysis of HSP70 from the 4 groups at 24 and 72 hours in cortex. B, Immunostaining of HSP70 (green) and DAPI (violet) in sham, saline, L‐glutamine, and L‐glutamine + Apoptozole group. Bar = 50 μm/20 μm. C, Bar graph showed SOD, GSH activity, and MDA level of the 4 groups. D, Western blot analysis of Nrf2 and NF‐κB (p65) expression at 24 hours of the 4 groups. β‐actin or p65 was used as loading control, respectively. N = 5‐6 per group. Data are presented as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001