Figure 3.

L‐glutamine increased HSP70 in astrocytes and endothelial cells of stroke mouse brain and reduced neuronal apoptosis. A, Double immunofluorescent staining of HSP70 (green)/NeuN (red), HSP70 (green)/Iba‐1 (red), HSP70 (green)/GFAP (red), and HSP70 (green)/CD31 (red) in the saline and L‐glutamine groups. Bar = 50 μm. B, Statistical analysis of HSP70⁺/NeuN⁺, HSP70⁺/Iba‐1⁺, HSP70⁺/GFAP⁺, and HSP70⁺/CD31⁺ cells in the saline and L‐glutamine groups. C, Immunofluorescence staining showed the spatial relationship between HSP70⁺/GFAP⁺astrocytes and HSP70⁺/CD31⁺ endothelial cells after MCAO. Bar = 50 μm. D. TUNEL staining showed neuronal apoptosis in the 3 groups. Bar = 50 μm. N = 5 per group. Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001