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. Author manuscript; available in PMC: 2019 Aug 19.
Published in final edited form as: J Vis Exp. 2019 Mar 15;(145):10.3791/59299. doi: 10.3791/59299

Figure 1: Selection and culture of pupae for dissection.

Figure 1:

(A) Wandering third larval instar (L3) larvae and pupae locate along the sides of healthy Drosophila cultures. (B) Pre-pupae can be identified by their translucent white color as pigment has yet to be generated in the protective pupal case. Anterior-posterior and dorsal-ventral axis of the pupa are shown in blue. A damp bamboo splint is used to dislodge and pick pre-pupae from the vial walls. (C) Pupae are placed inside 1.5 mL microcentrifuge tubes that are labelled appropriately (genotype, date of collection, and time of collection) and (D) cultured inside a humidified chamber assembled from an empty pipette-tip box. Humidity is maintained by placing a piece of damp tissue inside the box.