Table 2B.

EHV-1 genes display enhanced expression in the absence of the UL4 gene

	Fold Increasea			Avg Fold Increased
Gene	Exp 1b	Exp 2c	Exp 3c		SEMe	Name	Functionf	HSV
ORF38	1.54	1.45	1.76	1.60	0.155	UL38	Thymidine kinase	UL23
IR3	1.52	1.17	2.03	1.60	0.430	IR3	microRNA	none
ORF39	1.67	1.41	1.78	1.60	0.185	UL39	gH	UL22
ORF18	1.89	1.54	1.61	1.58	0.035	UL18	DNA process	UL42
ORF45	1.59	1.42	1.73	1.57	0.155	UL45	Tegument req. for cleavage/packaging	UL17
ORF74	1.33	1.45	1.59	1.52	0.070	US7	gE	US8
ORF17	1.10	1.61	1.43	1.52	0.090	UL17	Membrane	UL43
ORF46	1.93	1.38	1.63	1.50	0.125	UL46	Capsid-assoc. req. for cleavage/packaging	UL16
ORF49	1.77	1.35	1.66	1.50	0.155	UL49	Tyr protein kinase	UL13
ORF75	1.96	1.40	1.60	1.50	0.100	US8	“10K”	none
ORF34	1.96	1.45	1.54	1.49	0.045	UL34	??	none
ORF29	1.61	1.37	1.61	1.49	0.120	UL29	Req. for envelopment	UL31
ORF50	1.69	1.38	1.59	1.49	0.105	UL50	Alkaline Dnase	UL12
ORF28	1.56	1.35	1.59	1.47	0.120	UL28	Cleavage/packaging	UL32
ORF8	1.34	1.32	1.61	1.47	0.145	UL8	Tegument	UL51
ORF27	1.29	1.27	1.64	1.46	0.185	UL27	Packaging	UL33
ORF24	1.53	1.37	1.54	1.46	0.085	UL24	ICP1/2 tegument	UL36
ORF69	1.23	1.44	1.45	1.45	0.005	US2	Protein kinase	US3
ORF25	2.18	1.27	1.61	1.44	0.170	UL25	Capsid	UL35
ORF20	1.93	1.24	1.64	1.44	0.200	UL20	RR2	UL40
ORF7	1.19	1.27	1.56	1.42	0.145	UL7	Helicase/primase	UL52
ORF26	1.66	1.30	1.52	1.41	0.110	UL26	Membrane	UL34
ORF5	1.32	1.31	1.46	1.39	0.075	UL5	EICP27	UL54
ORF51	2.13	1.21	1.56	1.38	0.175	UL51	Virion and envelopment	UL11
ORF31	1.57	1.36	1.40	1.38	0.020	UL31	ICP8 ssDNA-binding protein	UL29
ORF48	1.22	1.17	1.58	1.38	0.205	UL48	Tegument	UL14
ORF21	1.31	1.24	1.47	1.36	0.115	UL21	RR1	UL39
ORF67	1.91	1.35	1.36	1.36	0.005	IR6	Virion forms filaments	none
IR4/UL3	1.31	1.18	1.45	1.32	0.135		Hybrid	none
ORF32	1.09	1.19	1.30	1.24	0.055	UL32	Cleavage/packaging	UL28
ORF64.1	0.74	0.95	1.50	1.23	0.275	IR1	IE	ICP4
ORF76	0.94	1.14	1.29	1.22	0.075	US9	Tegument	US9
ORF63	1.35	1.00	1.36	1.18	0.180	UL63	EICP0	IE110
ORF65	1.13	1.00	1.25	1.13	0.125	IR4	EICP22	US1
ORF1g	−1.06	0.69	1.50	1.10	0.405	UL1	MHC-I downregulation, immune evasion	UL56
Actin	0.80	1.01	1.00	1.00	0.005
GAPDH	1.00	1.00	1.00	1.00	0.000
ORF4h	−0.10	0.17	0.38	0.27	0.105	UL4	Inhibitory protein req. for DIP generation	UL55

^aFold increase ΔUL4 EHV-1 vs. wild-type EHV-1. Data are from three independent experiments (Exp)

^bInfection MOI 5

^cInfection MOI 10

^dAverage fold increase for Exps 2 & 3

^eStandard error from mean Exps 2 & 3

^fFunction or location is assigned based on sequence analysis (Telford et al., 1992)

^gORF1 deleted in both wild-type EHV-1 and ΔUL4 EHV-1

^hORF4 deleted in ΔUL4 EHV-1 but present in wild-type EHV-1