Figure 8.

Quantification of std-AAV6-9-Mediated GFP-Positive Cells in the Contralateral Hippocampus

(A) Representative coronal brain section showing GFP distribution after unilateral injection of std-AAV6-9 vectors (2 μL) into the ipsilateral hemisphere and PBS (2 μL) into the contralateral hemisphere of C57BL/6 mice. Images were captured by a Leica SP8 confocal microscope at 10× magnification. Scale bar 1 mm. (B) Quantification of GFP-positive cells visible enumerated in CA1, CA2, and CA3 and in DG in the ipsilateral hemisphere. CA2 (n = 3; p = 0.0236) and DG (n = 3; p = 0.0227). (C) Quantification of GFP-positive cells visible enumerated in CA1, CA2, and CA3 and in DG in the contralateral hemisphere. CA1 (n = 3; p = 0.0100), CA2 (n = 3; p = 0.0036), CA3 (n = 3; p = 0.0004), and DG (n = 3; p = 0.0029). Data were analyzed by an unpaired Student’s t with Mann-Whitney test and are expressed as percentages of GFP⁺ cells. Results were considered statistically significant with *p < 0.05 and levels of significance are indicated as follows: *p < 0.05, **p < 0.01, and ***p < 0.001. Data are presented as mean ± SEM. Images were by Leica DM6000 B fluorescence at 63× magnification. Scale bar 50 μm.