Figure 4.

G-Rg1 reduces the PA-induced increases in the mRNA and protein expression levels of lipogenic genes. HepG2 cells were treated with 0.25 mM PA for 24 h and then cultivated with 40 or 80 μg/mL G-Rg1 for 6 h. Total proteins were extracted for western blotting (a), quantified by means of the intensities of their bands (b), and total RNA was isolated for qPCR analysis of SREBP-1c and FAS (c). The data represent the means ± SDs of three individual experiments; n=3; ∗P<0.05, ∗∗P<0.01, ∗∗∗P<0.001 (in comparison to the PA-treated model group).