Figure 5.

The G-Rg1-mediated reduction of lipid accumulation is associated with AMPK activation. HepG2 cells were treated with 0.25 mM PA for 24 h, then cultured with 10 μM Compound C for 1 h, and then cultivated with 40 or 80 μg/mL G-Rg1 for 6 h. The lipid accumulation was examined by Oil Red O staining (original magnification × 200), and the absorbance of the samples at 450 nm was measured using a microplate reader. (a) Effect of G-Rg1 on the intracellular TG levels in PA-treated HepG2 cells. (b) The phosphorylation of AMPK and ACCα was examined and quantified by western blotting. (c) The data are expressed as the means ± SDs; n=3; ∗P<0.05, ∗∗P<0.01, ∗∗∗P<0.001 (in comparison to the PA-treated model group).