Figure 6.

G-Rg1 attenuates PA-induced liver inflammation through the NF-κB pathway. HepG2 cells were treated with 0.25 mM PA for 24h and then cultivated with 40 or 80 μg/mL G-Rg1 for 6 h. The cell extracts were subjected to western blotting analysis. (a) The bands intensities were quantified using the Image Lab 6.0 software. (b) The expression levels of inflammatory genes were analyzed using qPCR analysis. (c) The nuclear translocation of NF-κB P65 was observed using laser confocal microscope (scale bars = 20 μm). (d) The data represent the means ± SDs of at least three individual experiments; ∗P<0.05, ∗∗P<0.01, ∗∗∗P<0.001 (in comparison with the PA-treated model group).