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RT-PCR and Western blotting analysis of the construct plasmid. (a) PCR amplification of target genes. M, DL2000 DNA marker. Lane 1, GM-CSF. Lane 2, IL-21. (b) RT-PCR analysis of the fusion GM-CSF and IL-21 in cells transfected with indicated plasmid. M, DL2000 DNA marker. Lane 1, GM-CSF. Lane 2, IL-21. Lane 3, GM-CSF/IL-21. (c) Western blotting analysis of MB49 cells transfected with Vector and PT-GM-CSF /IL-21plasmid.
