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GM-CSF/IL-21 enhanced the infiltration by CD4 + and CD8 + T cells and upregulated IL-12 and IFN-γ (P < 0.05). On day 19 after tumor injection, subcutaneous tumor tissue was collected from each group, and CD4+ and CD8+ T cells were detected by histology and immunohistochemistry (P < 0.05). Magnification, 200×.
