Figure 6.

Suppression of HIF-1α expression affected P4HB expression in GC cells. (a, b) GFP fluorescence and RT-PCR showing the efficiency of HIF-1α knockdown compared with that of the scrambled control in GC cells; ∗∗: P < 0.01. (b) GC cells were infected with shRNA-HIF-1α-II or LV-control for 24 h. P4HB expression levels were determined by western blot and RT-PCR. ∗∗: P < 0.01. (c, d) GC cells were transfected with HIF-1α siRNA; P4HB protein and mRNA levels were determined by western blot and RT-PCR, respectively.