Skip to main content
. 2019 Jul 28;2019:2596914. doi: 10.1155/2019/2596914

Table 1.

Primer sequences of genes for quantitative real-time PCR.

Symbol Primer Sequence (5′-3′) Amplicon size
Bta-miR-124a R-T: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCTTGGC
F: CGCGTAAGGCACGCGGTGAAT
R: ATCCAGTGCAGGGTCCGAGG
U6 R-T: CGCTTCACGAATTTGCGTGTCAT
F: GCTTCGGCAGCACATATACTAAAAT
R: CGCTTCACGAATTTGCGTGTCAT
PECR F: CCAGCAGTGGAGTAAGGATCAATAGTG 181bp
R: GCAGGAGACAGCAGGAAGCATAC
GADPH F: CGGCACAGTCAAGGCAGAGAAC 116bp
R: CCACATACTCAGCACCAGCATCAC
ELOVL2 F: GGCTGCCTCATCTTCCAGTCTTC 169bp
R: TTACTCCGTTCGCTGTGCTGAAG

Note: The genes in the above table are all from dairy cow species.