Table 1.
Purification steps | Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Fold purification | Yield (%) |
---|---|---|---|---|---|
Crude | 34391.97 | 1712.04 | 20.08 | – | 100 |
Ammonium Sulphate (0–90%) | 30459.17 | 515.45 | 59.09 | 2.94 | 88.56 |
DEAE Cellulose | 19302 | 18.6 | 1037.74 | 51.68 | 56.12 |
Sephadex G-200 | 7743 | 3.984 | 1943.52 | 96.78 | 22.51 |