Table 2.

Peptide sequences of both subunits obtained by ESI/MS and analyzed by Mascot Software. The peptide sequences matched with subtilisin-like serine protease pepD from Aspergillus niger strain CBS 513.88 (A2QTZ2) with 100% similarity.

Purified protease	Peptide sequence	Sequence coverage with A2QTZ2 (%)
Subunit 1	TIGGKTYGVSKNAHLLSVK	30.04
	HDHVAYVEQDQVWYLDTLVTERR
	ASLAYNAAGGEHVDDVGHGTHVAGTIGGK
	MAFLKR
	VENAFDEGVLSCVAAGNENR
	TERRAPWGLGSISHR
	ATEQVPHPTIQTIPGKYIVTFK
	ENAFDEGVLSCVAAGNENRDAAR
Subunit 2	VFVGESSSTSVILDGFNWAANDIVSK	30.7
	HDHVAYVEQDQVWYLDTLVTERR
	ASLAYNAAGGEHVDDVGHGTHVAGTIGGK
	TYRIANFAGYAGSFDEK
	MAFLKR
	LATRNAVTNVAGSPNLLAYNGNSGVSK