Effect of NanoSOD on genes modulating fatty acid metabolism and hepatic steatosis. Markers of fatty acid metabolism (A) were assessed in liver samples by real-time PCR. Hepatic lipid accumulation in CD (B), HF (C), and HF+Nano (D) was analyzed using hematoxylin and eosin staining, while hepatic triglycerides (E) were determined using gas chromatography. All mRNA values were normalized to 18S. Values are expressed as mean ± SEM of 10-14 samples in each group. *P<0.05 & **P<0.01 vs CD; ^P<0.05 vs HF. CD, chow diet; HF, high fat diet; and Nano, NanoSOD.