Table 1.

Stimulation index four weeks post final immunization assessed using ELISA in sera of immunized mice

Group	Stimulation index ± SD
PBS	0.86±0.09 (d,e,f,g)
pVitro2neo-mcs	0.73±0.13 (d,e,f,g)
CpG-ODN	1.05±0.11 (e,f,g)
pVitro-SAG1	1.36±0.1 (a,b,f,g)
pVitro-GRA7	1.58±0.12 (a,b,c,g)
pVitro-SAG1-GRA7	1.93±0.15 (a,b,c,d,g)
pVitro-SAG1-GRA7 with CpG-ODN	2.84±0.18 (a,b,c,d,e,f)
P-value	0.001

Notes: Significant differences (P<0.05) in PBS group (a), pVitro2neo-mcs group (b), CpG-ODN group (c), pVitro-SAG1 group (d), pVitro-GRA7 group (e), pVitro-SAG1-GRA7 group (f) and pVitro-SAG1-GRA7 with CpG-ODN group (g).

Abbreviations: PBS, phosphate-buffered saline; SD, standard deviation; IgG, immunoglobulin G; ELISA, enzyme-linked immunosorbent assay.