Table 2.
Cytokine production in vaccinated mice splenocyte cultures four weeks post final immunization using ELISA (pg/mL)
| Group | IFN-γ (mean ± SD) | IL-10 (mean ± SD) |
|---|---|---|
| PBS | 67.75±3.83 (d,e,f,g) | 37.75±3.03 |
| pVitro2neo-mcs | 80.75±5.76 (d,e,f,g) | 42.75±2.38 |
| CpG-ODN | 100.75±9.54 (d,e,f,g) | 39±1.581 |
| pVitro-SAG1 | 571.25±24.72 (a,b,c,e,f,g) | 35.75±2.58 |
| pVitro-GRA7 | 315.75±18.15 (a,b,c,d,f,g) | 42.25±4.91 |
| pVitro-SAG1-GRA7 | 802.5±31.66 (a,b,c,d,e,g) | 39.5±5.4 |
| pVitro-SAG1-GRA7 with CpG-ODN | 973.75±66.91 (a,b,c,d,e,f) | 41.25±4.81 |
| P-value | 0.001 | 0.29 |
Notes: Significant differences (P<0.05) in PBS group (a), pVitro2neo-mcs group (b), CpG-ODN group (c), pVitro-SAG1 group (d), pVitro-GRA7 group (e), pVitro-SAG1-GRA7 group (f) and pVitro-SAG1-GRA7 with CpG-ODN group (g).
Abbreviations: PBS, phosphate buffered saline; SD, standard deviation; IFN-γ, gamma interferon; IL-10, interleukin-10; ELISA, enzyme-linked immunosorbent assay.