Table 1.
Strains used in this study.
| Strain | Description | Reference/Origin |
|---|---|---|
| E. coli | Cloning host. Conjugation host |
Invitrogen, USA (Williams et al., 1990) |
| Top10 | ||
| CA434 | ||
| C. difficile | Clinical RT 027 isolate | J. Brazier, Anaerobe Reference Laboratory, Cardiff, United Kingdom |
| R20291 | ||
| R20291ΔpyrEΔPaLocΔcdtR | Model strain for complementation | [8] |
| R20291ΔPaLoc | pyrE-restored mutant. | [8] |
| R20291ΔPaLocΔcdtR | pyrE-restored mutant. | [8] |
| R20291ΔPaLocΔcdtR*cdtR | cdtR-complemented mutant | [8] |
| R20291ΔPaLocΔcdtR*M120-cdtR | M120 cdtR complement | This study |
| R20291ΔPaLocΔcdtR*cdtR-his | 6xhis cdtR complement | This study |
| R20291ΔPaLocΔcdtR*cdtR D61A-his | 6xhis phospho-cdtR complement | This study |
| R20291ΔPaLocΔcdtR*D61E-his | 6xhis dephospho-cdtR complement | This study |
| DH1916 | Clinical RT 027 isolate | Val Hall |
| L2 (31,568) | Clinical RT 027 isolate | Ed Kujiper |
| L6 (5,108,111) | Clinical RT 027 isolate | Ed Kujiper |
| L8 (32,219) | Clinical RT 027 isolate | Ed Kujiper |
| L10 (2191) | Clinical RT 027 isolate | Ed Kujiper |
| L14 (60,902) | Clinical RT 027 isolate | Ed Kujiper |
| L16 (26,131) | Clinical RT 027 isolate | Ed Kujiper |
| M120 | Clinical RT 027 isolate | [18] |
| Wilcox 078 | Clinical RT 078 isolate | Mark Wilcox |
| EK23 (Type 078) | Clinical RT 078 isolate | Ed Kujiper |
| EK24 (CD2315) | Clinical RT 078 isolate | Ed Kujiper |
| EK26 (2016) | Clinical RT 078 isolate | Ed Kujiper |
| EK27 (7,004,578) | Clinical RT 078 isolate | Ed Kujiper |
| EK28 (7,009,825) | Clinical RT 078 isolate | Ed Kujiper |
| CL5499 | Clinical RT 078 isolate | Christina Rodriguez |
| CL5502 | Pig RT 078 isolate | Christina Rodriguez |
| CL5503 | Pig RT 078 isolate | Christina Rodriguez |
| CL5504 | Pig RT 078 isolate | Christina Rodriguez |
| CL5506 | Pig RT 078 isolate | Christina Rodriguez |
| CL5655 | Pig RT 078 isolate | Christina Rodriguez |
| CL5656 | Pig RT 078 isolate | Christina Rodriguez |
| CL5657 | Pig RT 078 isolate | Christina Rodriguez |
| CL5695 | Pig RT 078 isolate | Christina Rodriguez |
| CL5696 | Pig RT 078 isolate | Christina Rodriguez |
| CL5698 | Pig RT 078 isolate | Christina Rodriguez |
| CL6136 | Pig RT 078 isolate | Christina Rodriguez |